Programmable RNA methylation and demethylation using PUF RNA binding proteins

Abstract

New methods to control local RNA methylation are needed to elucidate the function of individual m⁶A. Here, fusion proteins between the programmable RNA binding protein PUF and the m⁶A demethylase FTO or METTL14 methyltransferase were designed. FTO-PUFs and METTL14-PUFs showed sequence-specific RNA demethylation and methylation activities, respectively.