Bioscience Biotechnology and Biochemistry · 2019 · 17 citations · 49 references
MedicineOncologyPharmacologyApoptosisCancer PharmacologyMolecular OncologyApoptosis In CancerApoptosis RegulationApoptotic SignalingBreast Cancer PharmacotherapyCytotoxic ChemotherapyPharmacodynamics Of Anticancer AgentsAnticancer ActivityApoptosis Signaling In CancerApoptotic Signaling In CancerCancer Cell ApoptosisDocosahexaenoic AcidProtein Kinase B SignalingTriple-negative Breast Cancer TherapyWestern Blot Analysis
ABSTRACT The current study aimed to investigate the antitumor and antiangiogenesis effects of apatinib in triple-negative breast cancer in vitro and also whether the combination of docosahexaenoic acid (DHA) and apatinib is more effective than apatinib monotherapy. The cell counting kit-8 assay was used to measure cell proliferation. Flow cytometry was utilized to determine the cell apoptosis rate. A wound healing assay was utilized to assess cell migration. Western blot analysis was carried out to determine the effects of apatinib and DHA on Bcl-2, BAX, cleaved caspase-3, caspase-3, phosphorylated protein kinase B (p-Akt), and Akt expression. DHA in combination with apatinib showed enhanced inhibitory effects on cell proliferation and migration compared with apatinib or DHA monotherapy. Meanwhile, DHA combined with apatinib strongly increased the cell apoptosis percentage. DHA was observed to enhance the antitumor and antiangiogenesis effects of apatinib via further downregulation of p-Akt expression. Abbreviations: FITC: fluorescein isothiocyanate; PI: propidium iodide
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Jin Li, Shukui Qin, Jianming Xu et al. · Journal of Clinical Oncology · 2016 · 997 citations
Medicine, Oncology, Pharmacology +15
XBP1 promotes triple-negative breast cancer by controlling the HIF1α pathway
Xi Chen, Dimitrios Iliopoulos, Qing Zhang et al. · Nature · 2014 · 808 citations · Full text
Medicine, Cancer Biology, Oncology +10