Complete Genome Sequence of Acidithiobacillus ferrooxidans YNTRS-40, a Strain of the Ferrous Iron- and Sulfur-Oxidizing Acidophile

Abstract

<i>Acidithiobacillus ferrooxidans</i> YNTRS-40 (<i>A. ferrooxidans</i>) is a chemolithoautotrophic aerobic bacterium isolated from Tengchong hot springs, Yunnan Province, China, with a broad growth pH range of 1.0-4.5. This study reports the genome sequence of this strain and the information of genes related to the adaptation of diverse stresses and the oxidation of ferrous iron and sulfur. Results showed that YNTRS-40 possesses chromosomal DNA (3,209,933-bp) and plasmid DNA (47,104-bp). The complete genome of 3,257,037-bp consists of 3,349 CDS genes comprising 6 rRNAs, 52 tRNAs, and 6 ncRNAs. There are many encoded genes associated with diverse stresses adaptation and ferrous iron and sulfur oxidation such as <i>rus</i> operon, <i>res</i> operon, <i>petI</i>, <i>petII</i>, <i>sqr</i>, <i>doxDA</i>, <i>cydAB</i>, and <i>cyoABCD</i>. This work will provide essential information for further application of <i>A. ferrooxidans</i> YNTRS-40 in industry.