m ⁶ A Rna Modification Modulates Gene Expression and Cancer-Related Pathways in Clear Cell Renal Cell Carcinoma

Abstract

<b>Aim:</b> To systematically profile the global m⁶A modification pattern in clear cell renal cell carcinoma (ccRCC). <b>Methods:</b> m⁶A modification patterns in ccRCC and normal tissues were described via m⁶A sequencing and RNA sequencing, followed by bioinformatics analysis. m⁶A-related RNAs were immunoprecipitated and validated by quantitative real-time PCR (qPCR). <b>Results:</b> In total, 6919 new m⁶A peaks appeared with the disappearance of 5020 peaks in ccRCC samples. The unique m⁶A-related genes in ccRCC were associated with cancer-related pathways. We identified differentially expressed mRNA transcripts with hyper-methylated or hypo-methylated m⁶A peaks in ccRCC. <b>Conclusion:</b> This study presented the first m⁶A transcriptome-wide map of human ccRCC, which may shed lights on possible mechanisms of m⁶A-mediated gene expression regulation.