Advanced Materials · 2019 · 12 citations · 0 references
EngineeringBioengineeringBiotechnologyBiosensorsMolecular BiotechnologyMolecular DiagnosticsCrispr-cas SystemsElectrochemical BiosensorsMicrofluidic BiosensorsMicroscale BiosensorsNucleic Acid BiosensorsRna-based DiagnosticsCrispr DiagnosticsCrispr-based BiosensorsCrispr-based Molecular DiagnosticsCrispr-cas13 SystemsElectrochemical Microfluidic BiosensorsMicrorna BiosensorsNucleic Acid AmplificationNucleic Acid Amplification-based Biosensors
In article number 1905311, Can Dincer and co-workers introduce the first CRISPR-powered electrochemical microfluidic biosensor (CRISPR-Biosensor), allowing nucleic-acid-amplification-free detection of microRNAs in isolated serum samples. Upon recognition of its target, the activated Cas13a cleaves reporter RNAs in its surroundings, resulting in a decrease of the measured electrochemical signals. The CRISPR-Biosensor offers a low-cost, easily scalable, specific, and target amplification-free (i.e., rapid and easy-to-use) tool for on-site nucleic acid diagnostics.