A Stability Indicating Reversed Phase HPLC Method for Estimation of <i>trans</i>-Resveratrol in Oral Capsules and Nanoliposomes

Abstract

Trans-resveratrol, a naturally occurring lipophilic polyphenol present in various foods, is well known for its therapeutic use. Owing to their exciting therapeutic potential, we developed a simple and precise stability indicating reverse-phase high performance liquid chromatography (RP-HPLC) method as per International Conference on Harmonization guidelines for quantitative analysis of trans-resveratrol in a commercial nutraceutical product and in-house nanoliposomes formulation. The chromatographic separation was attained on Phenomenex Luna C18 column (150 x 4.6 mm, 5 μm) at 30°C using UV detection at 306 nm. The optimized mobile phase consisted of acetonitrile/water (30:70, v/v) at the flowrate of 1.0 mL/min under isocratic mode of elution. The proposed method was found to be accurate, precise and linear with regression coefficient of 0.999 which could analyse the samples with as low as in nanograms with percent recovery in the acceptable range of 97.97 - 102.80 %. In addition, stress degradation study was also carried out wherein analyte peak was found to be well resolved. Hence, the present investigation confirmed that the developed method can be employed for the analysis of trans-resveratrol in different formulations, and also of huge benefit in careful selection of experimental conditions and its implication for storage and formulation development.