PubMed · 1971 · 96 citations · 4 references
Life SciencesBiochemistryCarbohydrate BiochemistryCarbohydrate ChemistryProtein BiochemistryCarbohydrate-protein InteractionsGlycan StructurePolysaccharide StructureProtein GlycoengineeringProtein StructureAldehyde OxidationAldehydesAmino Acid Side Chain ChemistryAmino Protecting GroupsModification Of PolysaccharidesOxidationPolysaccharide-protein InteractionsProtein-polysaccharide Interactions
Proteins have been coupled to insoluble polysaccharides following periodate oxidation to produce reactive aldehyde groups which will react with side chain amino groups of proteins. The coupling is stabilized and unreacted aldehydes blocked, by reduction with sodium borohydride.
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