Polysaccharide-Based Lotus Seedpod Surface-Like Porous Microsphere with Precise and Controllable Micromorphology for Ultrarapid Hemostasis

Abstract

Rapid water absorption rate has become a bottleneck that limits ultrarapid hemostatic performance of hemostatic microspheres. Herein, we reported a "lotus seedpod surface-like" polysaccharide hemostatic microsphere (PHM) with "macropits on surface" morphology and "micropores in macropits" structure. Unique macropits on surface can promote the water absorption rate because they are advantageous to quickly guide blood into the micropores. Special micropores are internally connected with each other, which endows PHM₄ with high water absorption ratio. During the process of blood entering the micropores from micropits, the pore size decreases gradually. In this way, blood clotting factors could be rapidly concentrated. PHM₄ showed the highest water absorption rate (40.7 mL/s/cm²) and rapid hemostatic property in vivo (hemostatic time shortened from 210 to 45 s). Lotus seedpod surface-like PHMs are believed to have further clinical application as an effective hemostasis.