Association of Twelve Candidate Gene Polymorphisms with the Intramuscular Fat Content and Average Backfat Thickness of Chinese Suhuai Pigs

Abstract

The present study aimed to identify the molecular markers for genes that influence intramuscular fat content (IFC), but not average backfat thickness (ABT). A total of 330 Suhuai pigs were slaughtered, and measurements of IFC and ABT were obtained. Phenotypic and genetic correlations between IFC and ABT were calculated. Thirteen single nucleotide polymorphisms (SNPs) among 12 candidate genes for IFC were analyzed, including <i>FABP3</i>, <i>LIPE</i>, <i>IGF1</i>, <i>IGF2</i>, <i>LEP</i>, <i>LEPR</i>, <i>MC4R</i>, <i>PHKG1</i>, <i>RETN</i>, <i>RYR1</i>, <i>SCD,</i> and <i>UBE3C</i>. Associations of the evaluated SNPs with IFCIFC and ABT were performed. Our results showed that the means of IFC and ABT were 1.99 ± 0.03 % and 26.68 ± 0.28 mm, respectively. The coefficients of variation (CVs) of IFC and ABT were 31.21% and 19.36%, respectively. The phenotypic and genetic correlations between IFC and ABT were moderate. Only the <i>FABP3</i> (rs1110770079) was associated with IFC (<i>p</i> < 0.05) but not with ABT. Besides, there was a tendency for associations of <i>RYR1</i> (rs344435545) and <i>SCD</i> (rs80912566) with IFC (<i>p</i> < 0.1). Our results indicated that the <i>FABP3</i> (rs1110770079) SNP could be used as a marker to improve IFC without changing ABT in the Suhuai pig breeding system.