Biomass-Derived Dialdehyde Cellulose Cross-linked Chitosan-Based Nanocomposite Hydrogel with Phytosynthesized Zinc Oxide Nanoparticles for Enhanced Curcumin Delivery and Bioactivity

Abstract

A sustainable biomass-based nanocomposite hydrogel was formulated, characterized, and applied for curcumin delivery. Phytosynthesized zinc oxide nanoparticles (ZnO NPs) employing musk melon (<i>Cucumis melo</i>) seed extract was embedded in the hydrogel matrices and cross-linked using Dialdehyde cellulose prepared from sugarcane (<i>Saccharum officinarum</i>) bagasse (SCB). Nanoparticle incorporation enhanced the hydrogel's swelling degree to 4048% at pH 4.0. Also, an improved tensile strength of 14.1 ± 0.32 MPa was exhibited by the nanocomposite hydrogel compared to 9.79 ± 0.76 MPa for the pure chitosan cellulose hydrogel. A curcumin loading efficiency of 89.68% with around 30% increased loading was exhibited for the nanocomposite hydrogel. A Fickian diffusion-controlled curcumin release mechanism with maximum release at pH 7.4 was obtained. The synergistic effect on the antimicrobial activity was exhibited against <i>Staphylococcus aureus</i> and <i>Trichophyton rubrum</i>. The in vitro cytotoxicity studies employing L929 cells and A431 cells demonstrated good biocompatibility and enhanced anticancer activity of the curcumin-loaded green nanocomposite hydrogel compared to pure curcumin.