Cytotoxic Indolyl Diketopiperazines from the <i>Aspergillus</i> sp. GZWMJZ-258, Endophytic with the Medicinal and Edible Plant <i>Garcinia multiflora</i>

Abstract

Two new indolyl diketopiperazines, gartryprostatins A and B (<b>1</b> and <b>2</b>), with an unusual 2,3-furan-fused pyrano[2,3-<i>g</i>]pyrrolo[1″,2″:4',5']pyrazino[1',2':1,5]pyrrolo[2,3-<i>b</i>]indole nucleus, along with a new naturally occurring compound (gartryprostatin C, <b>3</b>) were isolated from the solid culture of <i>Aspergillus</i> sp. GZWMJZ-258, an endophyte from <i>Garcinia multiflora</i> (Guttiferae). The structures of compounds <b>1</b>-<b>3</b> were determined by nuclear magnetic resonance, mass spectrometry, Marfey's analysis of amino acids, and chemical calculation. Compounds <b>1</b>-<b>3</b> displayed selective inhibition on human FLT3-ITD mutant AML cell line, MV4-11, with IC₅₀ values of 7.2, 10.0, and 0.22 μM, respectively.