Lentibacillus lipolyticus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi)

Abstract

A Gram-stain-positive, aerobic, spore-forming, moderately halophilic bacterium, SSKP1-9^T, was isolated from traditional salted shrimp paste (<i>Ka-pi</i>) produced in Samut Sakhon Province, Thailand. This strain grew optimally at 37-40 °C, pH 7.0 and in the presence of 8-16 % (w/v) NaCl. The 16S rRNA gene sequence similarity values between strain SSKP1-9^T and <i>Lentibacillus juripiscarius</i> TISTR 1535^T and <i>Lentibacillus halophilus</i> TISTR 1549^T were 98.7 and 97.2 %, respectively. Based on 16S rRNA gene sequence similarity, strain SSKP1-9^T represents a distinct novel species, as shown by phenotypic traits, DNA-DNA hybridization and average nucleotide identity values. In addition, the whole-cell protein profile confirmed the novelty of the taxon. The genomic DNA G<i>+</i>C content was 44.6 mol%. The major isoprenoid quinone was MK-7. The cell-wall peptidoglycan contained <i>meso</i>-diaminopimelic acid. Polar lipid analysis revealed the presence of phosphatidylglycerol, diphosphatidylglycerol, four unidentified lipids, an unidentified phospholipid and an unidentified glycolipid. The major cellular fatty acids were anteiso-C_{15 : 0}, anteiso-C_{17 : 0} and iso-C_{16 : 0}. The results of phenotypic and chemotaxonomic characteristics and whole-genome analysis support that strain SSKP1-9^T represents a novel species of <i>Lentibacillus</i>, for which the name <i>Lentibacilluslipolyticus</i> sp. nov. is proposed. The type strain is SSKP1-9^T (=JCM 32625^T=TISTR 2597^T).