Antitumour effect of glucooligosaccharides obtained via hydrolysis of α-(1 → 3)-glucan from Fomitopsis betulina

Abstract

Novel α-(1 → 3)-glucooligosaccharides (α-(1 → 3)-GOS) were prepared by acid hydrolysis of α-(1→ 3)-glucan isolated from Fomitopsis betulina fruiting bodies and characterized. Their anti-cancer potential was evaluated in in vitro assays in a colon cancer cell model. The tested α-(1 → 3)-GOS showed antiproliferative (MTT assay) and pro-apoptotic (Annexin V-FITC and PI technique) features against colon cancer but not against normal epithelial colon cells. Additionally, we did not observe cytotoxic activity (neutral red and lactate dehydrogenase assays) of α-(1 → 3)-GOS against several types of normal cell lines. In the present study, we demonstrated the anticancer potential of α-(1 → 3)-GOS in a colon carcinoma model. The anti-tumour effect of α-(1 → 3)-GOS is related with induction of apoptosis. Based on these results, we conclude that α-(1 → 3)-GOS may be considered as a dietary or therapeutic agent with an ability to inhibit the growth of cancer cells.