Silicon application positively alters pollen grain area, osmoregulation and antioxidant enzyme activities in wheat plants under water deficit conditions

Abstract

Role of exogenously-applied silicon (Si) on antioxidant enzyme activities was investigated in wheat under drought stress using a completely randomized factorial design with four replications. Drought stress significantly enhanced activities of ascorbate peroxidase, peroxidase, superoxide dismutase and catalase, and elevated accumulation of osmotically active molecules, soluble sugars and proline. Si application further enhanced activities of enzymes involved in oxidative defense system and accumulation of osmotically active molecules in drought-stressed plants. Under drought stress conditions, water shortage decreased protein content in all cultivars; however, application of Si increased it. Pollen area ratio was lower than 1 for cvs. Shiraz and Marvdasht under drought, but greater than 1 for cvs. Chamran and Sirvan. Water-limited regimes resulted in decreased leaf Ψw in all cultivars, but Si supply was effective in improving Ψw under water-limited regimes. Water shortage increased leaf K, Mg, and Ca concentrations. Under drought stress, Si-treated plants had higher K concentration than the none-treated plants.