NADPH-dependent extracellular superoxide production is vital to photophysiology in the marine diatom <i>Thalassiosira oceanica</i>

Abstract

Significance Superoxide and other reactive oxygen species (ROS) are commonly regarded as harmful progenitors of biological stress and death, but this view has been changing. Indeed, many phytoplankton actively generate extracellular superoxide under ideal growth conditions for reasons that are mysterious. Results from this study suggest that extracellular superoxide production by the marine diatom Thalassiosira oceanica may promote photosynthetic health by modulating the oxidation state of the cellular NADP + /NADPH pool. The key enzyme implicated in this process is present in other representative marine phytoplankton and global ocean metagenomes. Overall, these findings transform the perceived role of superoxide in the health and functioning of phytoplankton and present implications for redox balance, biogeochemistry, and ecology in the future ocean.