Angewandte Chemie International Edition · 2000 · 17 citations · 0 references
Life SciencesBiochemistryChemistryAnalytical ChemistryCarbohydrate BiochemistryCarbohydrate ChemistryCarbohydrate SpectroscopyCarbohydrate StructureGlycan AnalysisGlycan StructureConfigurational AnalysisDisaccharide AnalysisGlycosidic Linkage AnalysisMultidimensional Nmr SpectroscopyOligosaccharide AnalysisOligosaccharide CharacterizationStereochemistry Of Glycosides
With no prior knowledge of the identity of single components, a complete assignment of a bioactive ligand from a mixture of 15 carbohydrate ligands can be accomplished by utilizing NMR spectroscopy. A 3D-TOCSY-(transfer)NOESY experiment delivered essential information for the critical assignment of the glycosidic linkage type of the bioactive disaccharide (see scheme).