mBio · 2018 · 131 citations · 53 references
AgricultureGeneticsPlant BiologyPlant GeneticsPlant GenomicsPlant-microbe InteractionsBacterial Pathogenesis In PlantsBacterial SurvivalPathogen RecognitionPlant Immune ResponsesPlant-pathogen InteractionsRhizosphere ColonizationHost Defense ResponsesHost Resistance GenesMicrobial Evasion Of Host DefensesPathogen Resistance GenesPathogenesis Of Plant-associated BacteriaResistance Induction By Beneficial MicrobesRhizosphere FitnessRhizosphere Microbial Interactions
While rhizosphere bacteria hold the potential to improve plant health and fitness, little is known about the bacterial genes required to evade host immunity. Using a model system consisting of Arabidopsis and a beneficial Pseudomonas sp. isolate, we identified bacterial genes required for both rhizosphere fitness and for evading host immune responses. This work advances our understanding of how evasion of host defenses contributes to survival in the rhizosphere.
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Site-directed mutagenesis by overlap extension using the polymerase chain reaction
Steffan N. Ho, Henry D. Hunt, Robert M. Horton et al. · Gene · 1989 · 7.5K citations
Microtiter Dish Biofilm Formation Assay
George A. O’Toole · Journal of Visualized Experiments · 2011 · 2.2K citations · Full text