Concepedia

Design and evaluation of engineered protein biosensors for live-cell imaging of EGFR phosphorylation

Karthik Tiruthani, Adam Mischler, Shoeb Ahmed, Jessica Mahinthakumar, Jason M. Haugh, Balaji M. Rao

Science Signaling · 2019 · 16 citations · 31 references

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Concepts

Abstract

(SPY1148). This approach of integrating theoretical considerations with protein engineering strategies can be generalized to design and evaluate suitable biosensors for various phospho-specific targets.

References

31

SH2 domains recognize specific phosphopeptide sequences

Cell · 1993 · 2.7K citations

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SH2 domains prevent tyrosine dephosphorylation of the EGF receptor: identification of Tyr992 as the high‐affinity binding site for SH2 domains of phospholipase C gamma.

Daniela Rotin, Ben Margolis, Moosa Mohammadi et al. · The EMBO Journal · 1992 · 338 citations · Full text

, , +17

Epidermal growth factor receptor-mediated cell motility: phospholipase C activity is required, but mitogen-activated protein kinase activity is not sufficient for induced cell movement.

P Chen, Haihua Xie, Mahendran Sekar et al. · The Journal of Cell Biology · 1994 · 328 citations · Full text

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The role of tyrosine kinase activity in endocytosis, compartmentation, and down-regulation of the epidermal growth factor receptor

H Wiley, John J. Herbst, Bradley J. Walsh et al. · Journal of Biological Chemistry · 1991 · 287 citations · Full text

, , +17

Variants of 3T3 cells lacking mitogenic response to epidermal growth factor.

Rebecca M. Pruss, Harvey R. Herschman · Proceedings of the National Academy of Sciences · 1977 · 278 citations · Full text

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