Concepedia

Measuring proteins in H<sub>2</sub>O with 2D-IR spectroscopy

Samantha Hume, Gordon Hithell, Gregory M. Greetham, Paul M. Donaldson, Michael Towrie, Anthony W. Parker, Matthew J. Baker, Neil T. Hunt

Chemical Science · 2019 · 55 citations · 27 references

DOIFull text

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Concepts

Abstract

2D-IR spectroscopy is used to measure protein amide I bands in water, avoiding the need for deuteration. We show that H/D exchange affects protein vibrational relaxation dynamics and that the ability to perform 2D-IR in water enables blood serum protein analysis.

References

27

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