Phytochemical screening and wound healing activity of Telephium imperati (L.) in rats

Abstract

Telephium imperati (L) is a Caryophyllaceae species and is traditionally used in Morocco for its wound healing properties. In the present study the aim was to evaluate the wound healing activity of 5% ointment of hydroalcoholic extract of Telephium imperati (L.) on Wistar rat's skin Phytochemical screening and thin-layer chromatography were carried out to identify possible compounds. Eighteen male Wistar Albinos rats (150–180 g) were divided into three groups. The test group treated with experimental ointment, control group treated with Vaseline and the reference group treated with standard drug (MADECASSOL®). Burn wounds were induced on dorsal area of the rats body. The wound area measurement was carried out every 5 days during 55 days; and also histopathology evaluation was performed 24 h after burn wound creation, 15th, 25th and 55th days. The results of physicochemical screening and thin-layer chromatography showed the presence of saponins, flavonoid and Quercetin. Significant wound healing activity was observed with topical application of Telephium imperati (L.) hydroalcoholic extract. Reduction of the wound area at the end of each treatment each was 95.5%, 97.5% and 75.75% for the test, reference and control groups, respectively. Moreover, the tissue histology of 5% ointment treated groups was most effect on the wound healing process with good fibroblasts proliferation, few inflammatory cells and well-organized collagen. The results of this study confirmed the traditional use of Telephium imperati (L) on wound healing management.