Ac/Ds Transposition Activity in Transgenic Rice Population and DNA Flanking Sequence of Ds Insertion Sites

Abstract

The Agrobacterium mediated transgenic rice (Oryza sativa L.) population with inserts of maize transposon Activator/Dissociation (Ac/Ds) was investigated. DNA sequences flanking the T-DNA were analyzed with inverse PCR. Results showed that 65.4% of the T-DNA was integrated in different locations of rice genome, and some T-DNA flanking sequences were located on certain chromosomes. A number of T-DNA was found to have inserted into protein codign regions. In order to induce transposition of the inserted Ds elements, 354 crosses ofo Ac*Ds and Ds*Ac were constructed. The excision frequency of Ds element trans-activated by Ac transposase was 22.7% in the F_(2) populations, and the transposition was confirmed with analyses of DNA sequences flanking the Ds elements.