ACS Applied Materials & Interfaces · 2019 · 74 citations · 15 references
Natural SciencesBiochemistryBiophysicsBiomedical OpticsBiophotonicsMitochondrial BiologyFluorescence SpectroscopyInfrared SpectroscopyMitochondrial FunctionMitochondrial PhysiologyNear-infrared ImagingNear-infrared SpectroscopyBiomedical ApplicationsFluorescence Imaging ProbesFluorescent ProbesNear-infrared Fluorescence ImagingNear-infrared ProbesPh DependencePh-responsive SystemsPh-sensitive Probes
Mitochondria are crucial in the process of oxidative metabolism and apoptosis. Their morphology is greatly associated with the development of certain diseases. For specific and long-term imaging of mitochondrial morphology, we synthesized a new mitochondria-targeted near-infrared (NIR) fluorescent probe (TPE-Xan-In) by incorporating TPE with a NIR merocyanine skeleton (Xan-In). TPE-Xan-In displayed both absorption (660 nm) and emission peaks (743 nm) in the NIR region. Moreover, it showed aggregation-induced emission properties at neutral pH and specifically illuminated mitochondria with good biocompatibility, superior photostability, and high tolerance to mitochondrial membrane potential changes. With a pH-responsive unit, hydroxyl xanthene (Xan), the probe exhibited a pH-sensitive fluorescence emission in the range of pH 4.0-7.0, which indicated its potential in long-term tracking of pH and morphology changes of mitochondria in the biomedical research studies.
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Aggregation-induced emission of 1-methyl-1,2,3,4,5-pentaphenylsilole
Jingdong Luo, Zhiliang Xie, Jacky W. Y. Lam et al. · Chemical Communications · 2001 · 8.2K citations