Development of a Multiplexed Assay for Detection of <i>Leishmania donovani</i> and <i>Leishmania infantum</i> Protein Biomarkers in Urine Samples of Patients with Visceral Leishmaniasis

Abstract

, respectively. In contrast, a multiplexed assay designed to simultaneously detect all five leishmanial biomarkers markedly increased the assay sensitivity to 82.2%. These results validate the utility of leishmanial protein biomarkers found in the urine of VL patients as powerful tools for the development of an accurate diagnostic test for this disease.