Isolation and identification of proteolytic bacteria from pig sludge and protease activity determination

Abstract

Proteolytic bacteria were found in many environment, some were found in soil or mud. This research was conducted to find proteolytic bacteria from pig sludge that is place for pig wallowing. Bacteria isolation was carried out by streak plate method on nutrient agar media. Qualitative test of proteolytic bacteria used skim milk agar medium. The results of isolation obtained 10 bacterias and 2 of them have potential as proteolytic bacteria. Identification of proteolitic bacteria used the 16s rRNA method, result showed that bacterias are Bacillus pseudomycoides and Staphylococcus sciuri. Protease isolation was carried out under 4°C and under room temperature during centrifugation as separation process. Protease activity from Bacillus pseudomycoides was at 4°C as much as 0.094 U/mL and was 0.082 U/mL at room temperature, while from Staphylococcus sciuri was at 4°C as much as 0.120 U/mL and was 0.098 U/mL at room temperature.