Statistical Optimization of Process Parameters by Central Composite Design (CCD) for an Enhanced Production of L-asparaginase by <i>Myroides gitamensis</i> BSH-3, a Novel Species.

Abstract

BACKGROUND: The present study focused on the production of L-asparaginase using Solid State Fermentation (SSF) by METHODS: . Central Composite Design (CCD) was also employed successively to optimize the multiple parameters at a time and their results were compared. RESULTS: . Upon validation, the obtained results proved that there was a good relation existing between the experimental and the predicted model (p<0.05). L-asparaginase activity was enhanced in statistical method up to 3.4 folds compared to that of classical method. CONCLUSION: Utilization of wheat bran as a low cost carbon source in SSF for the production of L-asparaginase enzyme makes the process economical and in turn reduces the environmental pollution by biotransformation to commercially useful bio product.