Antioxidant and anticholinesterase potential of Ferulago cassia with farther bio-guided isolation of active coumarin constituents

Abstract

Ferulago species are utilized as flavor, sedative, tonic, aphrodisiac, peptic, immunostimulant, antibronchitis, vermicidal, carminative and for the curation of cancers and skin disorders in Middle Eastern region. The presented research reports anticholinesterase and antioxidant capacities of aerial parts, fruits, flowers, roots extracts and isolated coumarins of roots from of Ferulago cassia Boiss (peucedanol (1), suberosin (2), grandivitinol (3) and umbelliferone (4)). The TBA antioxidant activity results of some samples were strong when compared the references rutin, propyl gallate and chlorogenic acid. The highest phenolics level and DPPH antioxidant capacity were determined in fraction from root and fruit. Among different studied extract fractions, the CH2Cl2 fractions, especially root and fruit, has been detected to indicate considerable acetylcholinesterase (AChE) (53.24%) and butyrylcholinesterase (BuChE) (96.56%) inhibition at 20 μg/mL, respectively. Peucedanol (76.22%) and suberosin (71.67%) indicated strong inhibition towards BuChE. The umbelliferone (61.09%) exhibitted strong inhibition towards AChE at 20 μg/mL. F. cassia can be used a potency for pharmaceutical products which have antioxidant and anticholinesterase activity.