Membrane potential (V_mem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation

Abstract

We found that EStim caused increased AT-MSC proliferation that corresponded to V_mem depolarization and increased osteogenic differentiation that corresponded to V_mem hyperpolarization. Taken together, this shows that V_mem changes associated with EStim induced cell proliferation and differentiation can be accurately tracked during these important cell functions. Using this tool to monitor V_mem changes associated with these important cell behaviors we hope to learn more about how these electrochemical cues regulate cell function with the ultimate goal of developing new EStim based treatments capable of controlling healing and regeneration.