RSC Advances · 2019 · 30 citations · 28 references
MedicineMolecular BiologyOncologyBiosensorsMolecular DiagnosticsMolecular OncologyAssay ValidationBiomarkers In PathologyCancer BiomarkersCancer BiotechnologyCancer Molecular DiagnosticsMolecular BiomarkersAptamer-based BiosensingAptamer-based DetectionAptamersBiomarkersEarly DiagnosisMaldi-tof IdentificationMaldi-tof Mass SpectrometrySelex
of 128 ± 26.3 nM, 149 ± 23.6 nM, 232 ± 44.2 nM, 202 ± 25.6 nM, respectively. These generated aptamers showed higher specificities toward their target serum by differentiating normal serum but closely related other cancer serums. The selected four high affinity DNA aptamers were further applied to the development based on qPCR method for the early detection of gastric cancer. In addition, we performed MALDI-TOF MS followed by secondary peptide sequencing MS analysis for the identification of the aptamer binding proteins. Among these potential biomarkers, APOA1, APOA4, PARD3, Importin subunit alpha-1 showed a relatively high score probability. Therefore, these four ssDNA aptamers generated in our study could be a promising molecular probe for gastric cancer diagnosis.
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In vitro selection of RNA molecules that bind specific ligands
Andrew D. Ellington, Jack W. Szostak · Nature · 1990 · 9.7K citations