Isolation, identification and characterization of pancreatic lipase inhibitors from Trigonella foenum-graecum seeds

Abstract

Activity-guided fractionation of the methanol extract of Trigonella foenum-graecum seeds furnished three flavone C-glycosides having pancreatic lipase inhibitory activity. Structures of these compounds were established as vicenin-1 (1), isoschaftoside (2) and schaftoside (3) by detail analysis of 1H, 13C NMR and MS data. These compounds showed percentage inhibition 60.3% (1), 33.8% (2) and 95.5% (3) at the concentration of 250 μg/ml and IC50 values of the inhibition were 207 μg/ml (1), 330 μg/ml (2) and 130 μg/ml (3). This is the first report of the isolation of lipase inhibitors from T. foenum-graecum seeds. Temperature dependency of 1H NMR of 3 was studied in detail.