Toxins · 2018 · 58 citations · 13 references
Life SciencesEntomologyMolecular BiologyCell SignalingInsect GeneticsInsect PhysiologyBacterial Virulence FactorsGrowth Factor SignalingInsect PathogensMicrobial Virulence FactorsPathogen Virulence FactorsVirulence Gene RegulationExtracellular DomainsExtracellular ReceptorsInsect Pathogen VirulenceInsect Pattern Recognition ReceptorsInsecticidal Crystal ProteinsNovel Insecticidal ProteinsPull-down AssaysVegetative Insecticidal Proteins
Vegetative insecticidal proteins (Vips), which are secreted by some Bacillus thuringiensis strains during vegetative growth, exhibit high virulence to many pests. Vip3A proteins have been used commercially both in some bio-insecticides and in transgenic crops; however, compared with insecticidal crystal proteins, the mechanism of action of Vip3A is still unclear. In this work, we indicated that the fibroblast growth factor receptor-like protein (Sf-FGFR) from the membrane of Sf9 cells could bind to Vip3Aa. The interaction between Vip3Aa and Sf-FGFR was confirmed by pull-down assays and dot blotting experiment in vitro. The binding affinity between Vip3Aa and extracellular regions of Sf-FGFR (GST-FGFR-N) was determined by microscale thermophoresis assay (MST). Moreover, Vip3Aa-Flag could be co-immunoprecipitated with Sf-FGFR-V5 ex vivo. Furthermore, knockdown of Sf-FGFR gene in Sf9 cells resulted in reducing the mortality of those cells to Vip3Aa. In summary, our data indicated that Sf-FGFR is a novel receptor for Vip3Aa.
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Mutation of the mouse klotho gene leads to a syndrome resembling ageing.
PubMed · 1997 · 66.6K citations
Protein-binding assays in biological liquids using microscale thermophoresis
Christoph J. Wienken, Philipp Baaske, Ulrich Rothbauer et al. · Nature Communications · 2010 · 1.1K citations · Full text
J Estruch, Gregory W. Warren, Martha A. Mullins et al. · Proceedings of the National Academy of Sciences · 1996 · 712 citations