Storing Loblolly Pine Pollen

Abstract

Abstract A series of controlled-pollination tests with fresh pollen and pollen stored for up to 10 years demonstrates the conditions required to maintain high pollen viability and vigor. During handling, freshly extracted pollen is highly resistant to exposure to temperatures up to 50°C for as long as 24 hours. Moisture content must be reduced to less than 10% prior to storage. It can then be held in a refrigerator at 3°C for up to 1 year in a container that maintains low moisture content. For longer storage, dried pollen should be placed in tightly closed glass or plastic bottles and held at -20°C. Pollen can be stored in vacuum-sealed ampules at 3°C for at least 10 years. South. J. Appl. For. 15(3):153-157.