Bioanalytical Workflow for Novel Scaffold Protein–Drug Conjugates: Quantitation of Total Centyrin Protein, Conjugated Centyrin and Free Payload for Centyrin–Drug Conjugate in Plasma and Tissue Samples Using Liquid Chromatography–Tandem Mass Spectrometry

Abstract

The methods were successfully applied to analysis of samples from mice to quantify the plasma and tissue concentrations. This same workflow can potentially be applied to other PDCs and site-specific antibody-drug conjugates.