Evidences of N₂O Emissions in Chloropicrin-Fumigated Soil

Abstract

The mechanism of N₂O production following chloropicrin (CP) fumigation was investigated in this study. Our results showed that CP fumigation increased N₂O production from 23 to 25 times in comparison with the control and significantly decreased the abundance of 16S rRNA and N-cycling functional genes. CP also decreased the soil bacterial diversity and caused a shift in the community composition. The N₂O emissions in fumigated soil were significantly correlated with soil environmental factors (NH₄⁺, dissolved amino acid, microbial biomass nitrogen, and NO₃^-) but were not correlated with the abundance of functional genes. Metatranscriptomes and dual-label ¹⁵N-¹⁸O isotope analysis revealed that CP fumigation inhibited the expression of gene families involved in N₂O production and sink processes and shifted the main pathway of N₂O production from nitrification to denitrification. These results provided useful information for environmental safety assessments of CP in China, to improve our understanding of the N-cycling pathways in fumigated soils.