Nano-TiO₂ Inhibits Development of the Central Nervous System and Its Mechanism in Offspring Mice

Abstract

Nano titanium dioxide (Nano-TiO₂) has been applied in food packaging systems and food additives, but it may cause potential neurotoxicity for human and animals. In our study, the effects of nano-TiO₂ exposure during pregnancy/lactation on the development of the central nervous system in offspring mice were examined and its molecular mechanism involving Rho family was investigated. Our findings showed that pregnancy/lactation exposure to nano-TiO₂ resulted in thinning of cerebral and cerebellar cortex, decrease in number of neurons per unit area of cerebrum, edema and nuclear condensation, dysplasia of neurites in hippocampal pyramidal cells, thinning in pyramidal cell layer in hippocampus, and decrease in learning and memory of offspring mice. Furthermore, expressions of Rac1 and Cdc42 involved in axon and dendritic development were decreased, whereas RhoA expression and ratio of RhoA/Rac1 were increased in offspring brain. It implies that exposure to nano-TiO₂ during pregnancy/lactation could result in brain retardation and cognitive impairment in offspring mice, which was closely related to alterations in the expression of Rho protein family. Therefore, application of nano-TiO₂ in daily life should be performed with caution.