ChemBioChem · 2018 · 45 citations · 18 references
Life SciencesBiochemistryCatalysisEnzymologyRedox BiochemistryCatalytic MechanismsEnzymatic CatalysisEnzyme CatalysisEnzyme KineticsPhotocatalysisRedox EnzymesAlcohol Dehydrogenase CatalysisAlcohol Dehydrogenase RegenerationAldehyde Dehydrogenase CatalysisCatalytic EfficiencyNad+-dependent EnzymesNad-dependent EnzymesNadh-dependent EnzymesStereospecific Reactions
Abstract A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD + into the enzyme‐active 1,4‐NADH to promote an alcohol dehydrogenase catalysed stereospecific reduction reaction. The catalytic turnover of all the reaction components was demonstrated. Factors influencing the efficiency of the overall system were identified.
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Photo-induced degradation of some flavins in aqueous solution
Wolfgang Hölzer, J. Shirdel, Peyman Zirak et al. · Chemical Physics · 2004 · 158 citations