ESIPT-based fluorescence probe for the rapid detection of peroxynitrite ‘AND’ biological thiols

Abstract

An ESIPT-based 'AND' logic fluorescence probe (GSH-ABAH) was developed for the simultaneous detection of ONOO- and biological thiols. GSH-ABAH was shown to have good cell permeability and with the addition of just SIN-1 (ONOO- donor) or GSH, no fluorescence response was observed in live cells. However, in the presence of both analytes GSH-ABAH could be used to image exogenous ONOO- 'AND' GSH added to RAW264.7 cells.