Scientifica · 2018 · 18 citations · 8 references
Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup. Many microbes, including <i>Bacillus megaterium</i>, have been found to be able to produce glucose isomerase. However, the number of studies of glucose isomerase production from <i>Bacillus megaterium</i> is limited. In this study, we establish the optimal medium components and culture conditions for <i>Bacillus megaterium</i> glucose isomerase production by evaluating the combined influence of multiple factors and different parameters via Plackett-Burman design and response surface methodology in Modde 5.0 software. The optimized conditions, which were experimentally confirmed as follows: D-xylose (1.116%), K<sub>2</sub>HPO<sub>4</sub> (0.2%), MgSO<sub>4</sub>·7H<sub>2</sub>O (0.1%), yeast extract (1.161%), peptone (1%), pH 7.0, inoculum size 20% (w/v), shaking 120 rpm at 36.528°C for 48 hours, give rise to production of highest activity of glucose isomerase (0.274 ± 0.003 U/mg biomass). These results provide additional important information for future development of large-scale glucose isomerase production by <i>Bacillus megaterium.</i>
8