One-pot two-strain system based on glucaric acid biosensor for rapid screening of myo-inositol oxygenase mutations and glucaric acid production in recombinant cells

A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

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Enzymatic Assembly of Overlapping DNA Fragments

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Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli

Tae Seok Moon, John E. Dueber, Eric Shiue et al. · Metabolic Engineering · 2010 · 314 citations · Full text

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