Concepedia

The Amino Acid Composition of Hemoglobin. V. The Preparation of Purified Hemoglobin Fractions by Chromatography on Cellulose Exchangers and Their Identification by Starch Gel Electrophoresis Using Tris-Borate-EDTA Buffer

Amoz I. Chernoff, N.M. Pettit, JO NORTHROP

Blood · 1965 · 35 citations · 12 references

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Abstract

Abstract The chromatographic separation of hemoglobin on two cellulose exchangers, CMC and DEAE, is discussed. Problems related to the use of these technics are described. In addition, our experience with the use of Tris-Borate-EDTA buffer (Smithies) for hemoglobin electrophoresis in starch gel is presented.

References

12

Chromatography of Proteins. I. Cellulose Ion-exchange Adsorbents

Elbert A. Peterson, Herbert A. Sober · Journal of the American Chemical Society · 1956 · 1.3K citations

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Observations on the Chromatographic Heterogeneity of Normal Adult and Fetal Human Hemoglobin: A Study of the Effects of Crystallization and Chromatography on the Heterogeneity and Isoleucine Content

David W. Allen, W. A. Schroeder, Joan Z. Balog · Journal of the American Chemical Society · 1958 · 438 citations

, , +8

Studies on the heterogeneity of hemoglobin

T. H. J. Huisman, C.A. Meyering · Clinica Chimica Acta · 1960 · 176 citations

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Hemoglobin FI, an acetyl-containing hemoglobin

W. A. Schroeder, Genji Matsuda, William D. Fenninger · Biochimica et Biophysica Acta · 1962 · 168 citations

, , +9

Studies on the heterogeneity of hemoglobin. V. Binding of hemoglobin with oxidized glutathione.

Titus H.J. Huisman, Andrée M. Dozy · PubMed · 1962 · 157 citations

, , +11