Centella Asiatica Increased the Body Length Through the Modulation of Antioxidant in Rotenone-Induced Zebrafish Larvae

Abstract

Centella asiatica (CA) is herbal medicine that used as traditional medicine including ayurvedic theraphy since hundreds years ago. This herb containa of pentacyclic triterpenoids such as asiaticoside, madecassoside, Asiatic acid and brahmoside that proved had anti-oxidant and anti-inflammatory properties. This research aims to know the effect of ethanolic extract of CA extract against the length of rotenone-induced zebrafish larvae through the free radicals.mechanism. This research used zebrafish larvae until 6 dpf that consists of 5 groups (controls, rotenon 12.5 ppb on 2 hpf-3 dpf, and group treatment given rotenone 12.5 ppb 2 hpf-3 dpf and 5 µg/mL extract with long exposure to start 2 hpf to 4, 5 and 6 dpf respectively). The body length measured on 3-6 dpf using software Image Raster v 3.0 from optilab v 2.0. Malondialdehyde (MDA), superoxide Dismutase (SOD), catalase were measured by ELISA on 6 dpf. The results showed rotenon can inhibit the growth of length > 2 standard deviation (SD) and CA extract may increased ithe body in 6 dpf which correction value was 99.6%. CA extract significantly decreased the levels of MDA, and increased the level of SOD and catalase (p=0.000). Ethanol extract of Centella asiatica may increase in length through the modulation of oxidative stress.