Materials · 2018 · 24 citations · 18 references
EngineeringChemical EngineeringMaterials ScienceBioseparationsMembrane SciencePolymer ChemistryElectrostatics In BiomoleculesMembrane FabricationPolymer MembranesProtein AdsorptionProtein PurificationProtein SeparationAdsorption CapacityElectrospun MembranesElectrospun NanofibersLysozyme AdsorptionProtein Adsorption On MembranesProtein Purification By AdsorptionSelective AdsorptionSpecific Surface Area
In this work, electrospun poly(vinyl alcohol) (PVA) nanofiber membranes were functionalized by incorporating poly(methyl vinyl ether-alt-maleic anhydride) (poly(MVE/MA), PMA) for the selective adsorption of proteins. The capture performance was regulated by an optimizing buffer pH, PMA content, and protein concentration. Lysozyme was used as the model protein and a high adsorption capacity of 476.53 ± 19.48 was obtained at pH 6, owing to the electrostatic attraction between the negatively charged nanofibers and positively charged proteins. The large specific surface area, highly open porous structure, and abundant available carboxyl groups contributed to such high adsorption performance. Moreover, the nanofiber membranes exhibited good reusability and good selectivity for positively charged proteins. The obtained results can provide a promising method for the purification of proteins in small analytic devices.
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Kwan Woo Kim, Keun Hyung Lee, Myung Seob Khil et al. · Fibers and Polymers · 2004 · 150 citations
Natural Sciences, Materials Engineering, Materials Science +10