Crucial role of oxidative stress in bactericidal effect of parthenolide against <i>Xanthomonas oryzae</i> pv. <i>oryzae</i>

Abstract

Abstract BACKGROUND Xanthomonas oryzae pv. oryzae ( Xoo ) causes rice bacterial blight, which is one of the most devastating diseases on rice. Parthenolide (PTL) is a sesquiterpene lactone possessing multiple bioactivities. In the preliminary study, we found PTL can totally inhibit the growth of Xoo at 10 mg L −1 in vitro . In this study, we aim to further evaluate the anti‐bacterial activity of PTL against Xoo and discern the role of oxidative stress in its bactericidal effect. RESULTS PTL was effective against Xoo both in vitro and in vivo . PTL induced reactive oxygen species (ROS) accumulation in Xoo , leading to cell death, while exogenous catalase can fully abolish its bactericidal effect. PTL sensitivity of catalase deletion mutants of Xoo increased significantly compared with that of wild‐type Xoo strain. In addition, PTL treatment increased glutathione peroxidase activity and decreased glutathione (GSH) reductase activity in Xoo , but had no effect on its catalase and superoxide dismutase activities. Interestingly, PTL dramatically reduced the GSH level in Xoo , resulting in disturbed GSH/GSSG balance. Moreover, PTL rapidly reacted with GSH by a nucleophilic addition reaction. CONCLUSION PTL is a promising lead compound for developing bactericide against Xoo . PTL rapidly reacts with GSH, resulting in disturbed GSH/GSSG balance in Xoo , which causes ROS accumulation, leading to cell death. Oxidative stress plays a critical role in the bactericidal effect of PTL against Xoo . © 2018 Society of Chemical Industry