Concepedia

Development and validation of four one-step real-time RT-LAMP assays for specific detection of each dengue virus serotype

Benjamin López-Jimena, Michaël Bekaert, Mohammed A. Bakheit, Sieghard Frischmann, Pranav Patel, Etienne Simon‐Lorière, Louis Lambrechts, Veasna Duong, Philippe Dussart, Graham Harold,

PLoS neglected tropical diseases · 2018 · 71 citations · 45 references

DOIFull text

Open access

Abstract

We have shown a novel approach to design LAMP primers that makes use of fast growing sequence databases. The DENV1 and DENV2 assays were validated with viral RNA extracted clinical samples, showing very good performance parameters.

References

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