Publication | Closed Access
A colorimetric method for cysteine determination based on the peroxidase-like activity of ficin
37
Citations
38
References
2018
Year
Bioorganic ChemistryEngineeringLipid PeroxidationGreen ChemistryChemistryRedox BiologyOxidative StressBioanalysisBlue-colored OxtmbAnalytical ChemistryReaction IntermediateRedox ChemistryBiochemistryPhotochemistryCysteine DeterminationPeroxidase-like ActivityChromogenic ReactionReactive Oxygen SpecieBiomolecular EngineeringColorimetric MethodMedicine
H<sub>2</sub>O<sub>2</sub> could oxidize TMB to blue-colored oxTMB with catalysis by ficin. <sc>l</sc>-Cys competed with TMB for H<sub>2</sub>O<sub>2</sub> leading to fading of the chromogenic reaction.
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