Artificial Cells Nanomedicine and Biotechnology · 2018 · 41 citations · 35 references
EngineeringBioengineeringBiomedical EngineeringBiocompatibilityBiomaterialsTissue EngineeringBiomaterials For Tissue EngineeringLiver Tissue EngineeringNanobiomaterialsNanocarriers For TherapeuticsNanomedicineNanoparticle CharacterizationBiocompatibility ImprovementBiocompatible NanomaterialsBiocompatible NanoparticlesCross-linkingDecellularized TissuesNanomaterial BiocompatibilityNanoparticle BiocompatibilityNanoparticles
No ideal cross-linking agent has been identified for decellularized livers (DLs) yet. In this study, we evaluated structural improvements and biocompatibility of porcine DLs after cross-linking with silver nanoparticles (AgNPs). Porcine liver slices were decellularized and then loaded with AgNPs (100 nm) after optimization of the highest non-toxic concentration (5 µg/mL) using Human hepatocellular carcinoma (HepG2) and EAhy926 human endothelial cell lines. The cross-linking effect of AgNPs was evaluated and compared to that of glutaraldehyde and ethyl carbodiimide hydrochloride and N-hydroxysuccinimide. The results indicated that AgNPs improved the ultra-structure of DLs' collagen fibres with good porosity and increased DLs' resistance against in vitro degradation with good cytocompatibility. AgNPs decreased the host inflammatory reaction against implanted porcine DL slices in vivo and increased the polarization of M2 macrophages. Thus, structural and functional improvements of Porcine DLs could be achieved using AgNPs.
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The effect of pore size on cell adhesion in collagen-GAG scaffolds
Fergal J. O’Brien, Brendan A.C. Harley, Ioannis V. Yannas et al. · Biomaterials · 2004 · 1.3K citations · Full text
Matthew G. Haugh, Ciara M. Murphy, Ross C. McKiernan et al. · Tissue Engineering Part A · 2010 · 328 citations · Full text