Phytochemical Analysis and Hepatoprotective Effect of Hydroethanolic Extract of Stem Bark of Oroxylum indicum

Abstract

The present study was conducted to evaluate the hepatoprotective effect of the Hydroethanolic extract of the stem bark Oroxylum indicum against CCl4 induced hepatotoxicity. The hydroethanolic extract was prepared. Albino Wistar rats were taken for the study. The extract was prepared at a dose rate of 100mg/kg body weight, 300mg/kg body weight, 900mg/kg body weight. Carbon tetrachloride was used to induce hepatotoxicity. The extract was screened for phytochemical constituents. Enzyme activity of Alanine aminotransamenase, Alkaline phosphatase, Gamma-glutamyltransferase was tested and level of serum total bilirubin and total protein were analyzed. Results found that the shade-dried hydroethanolic bark extract has saponin, tannin, flavonoids, steroids, and glycosides. Biochemical analysis and Histopathological findings revealed the significant (p≤0.001) decrease of liver biomarkers in a dose-dependent manner resulting in the hepatoprotective effect of Oroxylum indicum over the carbon tetrachloride-induced hepatotoxicity and the results of the group treated with 900mg/kg body weight were comparable to as of control group. Hydroethanolic extract of O. indicum found to have hepatoprotective effect against CCl4 induced hepatotoxicity in Wistar rats.