Electrochemical Immune Analysis System for Gastric Cancer Biomarker Carcinoembryonic Antigen (CEA) Detection

Abstract

In the present work, carcinoembryonic antigen (CEA), a significant biomarker in gastric cancer diagnostics, was successfully detected by a desirably selective and sensitive electrochemical aptasensor with an electrocatalyst and nanocarrier, i.e., Pt/Au-diaminonaphthalene (DN)-graphene. The developed bioconjugate was captured onto the surface of the electrode via a “sandwich” strategy during the detection of CEA. The proposed method was demonstrated to be sensitive, as indicated by the improved electrochemical response, since the dendritic Pt/Au/DN-graphene showed peroxidase-mimic activity for the reduction of H 2 O 2 introduced into the electrolytic cell, thereby confirming its desirable catalysis capacity.