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Effect of the diet type and temperature on the<i>C. elegans</i>transcriptome

Eva Gómez‐Orte, Eric Cornes, Angelina Zheleva, Beatriz Sáenz‐Narciso, María de Toro, María Íñiguez, Rosario López, Begoña Ezcurra, Begoña Sacristán, Adolfo Sánchez-Blanco,

Oncotarget · 2017 · 58 citations · 50 references

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Life SciencesBiologyPhysiologyMetabolic RegulationMetabolismTranscriptomicsNematode PhysiologyPathway RegulationBacillus SubtilisDefense Response MechanismsDefense Response ModulationEscherichia ColiGlobal Gene RegulationPhysiological StatusTemperature Effects On GrowthTemperature-dependent Regulation

Abstract

// Eva Gómez-Orte 1 , Eric Cornes 2, 6 , Angelina Zheleva 1 , Beatriz Sáenz-Narciso 1 , María de Toro 1 , María Iñiguez 1 , Rosario López 1 , Juan-Félix San-Juan 3 , Begoña Ezcurra 1 , Begoña Sacristán 4 , Adolfo Sánchez-Blanco 5 , Julián Cerón 2 and Juan Cabello 1 1 CIBIR (Center for Biomedical Research of La Rioja), Logroño, 26006 La Rioja, Spain 2 Bellvitge Biomedical Research Institute - IDIBELL, L’Hospitalet de Llobregat, 08908 Barcelona, Spain 3 University of La Rioja, Logroño, 26006 La Rioja, Spain 4 Hospital San Pedro, Logroño, 26006 La Rioja, Spain 5 Department of Biology, University of Hartford, West Hartford, 06117 CT, USA 6 Present address: Institut Pasteur, 75015 Paris, France Correspondence to: Juan Cabello, email: juan.cabello@riojasalud.es Keywords: transcriptomic analysis; physiological response; growth temperature; diet; caenorhabditis elegans; Gerotarget Received: July 29, 2017      Accepted: October 27, 2017      Published: December 21, 2017 ABSTRACT The transcriptomes of model organisms have been defined under specific laboratory growth conditions. The standard protocol for Caenorhabditis elegans growth and maintenance is 20ºC on an Escherichia coli diet. Temperatures ranging from 15ºC to 25°C or feeding with other species of bacteria are considered physiological conditions, but the effect of these conditions on the worm transcriptome has not been well characterized. Here, we compare the global gene expression profile for the reference Caenorhabditis elegans strain (N2) grown at 15°C, 20°C, and 25°C on two different diets, Escherichia coli and Bacillus subtilis . When C. elegans were fed E. coli and the growth temperature was increased, we observed an enhancement of defense response pathways and down-regulation of genes associated with metabolic functions. However, when C. elegans were fed B. subtilis and the growth temperature was increased, the nematodes exhibited a decrease in defense response pathways and an enhancement of expression of genes associated with metabolic functions. Our results show that C. elegans undergo significant metabolic and defense response changes when the maintenance temperature fluctuates within the physiological range and that the degree of pathogenicity of the bacterial diet can further alter the worm transcriptome.