Influence of pH on adsorption of human immunoglobulin gamma, human serum albumin and horse myoglobin by commercial chromatographic materials designed for downstream processing of monoclonal antibodies

Abstract

Static adsorption capacity was measured for three different proteins: human immunoglobulin gamma, human serum albumin and horse skeletal muscle myoglobin. Eight commercial chromatographic materials were tested: FractoGel EMD SE HiCap (M), SP Sepharose Fast Flow, S Ceramic HyperD F, MabSelect, rProtein A Sepharose Fast Flow, Poros 50A High Capacity, ProSep –vA High Capacity and MEP HyperCel. The adsorption capacity was measured at pH values of 4.0, 5.0, 6.0, 7.0 and 8.0.