An optimized method for high quality DNA extraction from microalga Prototheca wickerhamii for genome sequencing

Abstract

A new method for DNA isolation from <i>Prototheca</i> algae is described. The method, whose protocol involves glass beads pulverization and cesium chloride (CsCl) density gradient centrifugation, was demonstrated superior over the other common assays in terms of DNA quantity and quality. The method is also the first to offer the possibility of preparation of DNA template suitable for whole genome sequencing of <i>Prototheca</i> spp.